Shoot Organogenesis and Plant Regeneration from Leaf Culture of Rehmannia elata L

For the regeneration of shoots, leaf segments of Rehmannia elata were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) (1, 2, or 4 mg L) with or without 1-naphthalene acetic acid (NAA) used at 0.1 or 0.5 mg L. Regenerated shoots were obtained from the leaf cultures at all BAP concentrations with or without both concentrations of NAA. However, the highest shoot regeneration frequency (92.23%) and shoot number per explant (4.03 shoots) were obtained on MS medium supplemented with BAP (4 mg L) and NAA (0.1 mg L) solidified with Gelrite (4 g L). The survival rate was ~70% when the rooted plants were hardened and transferred to soil. The continuous production of R. elataregenerated plants from leaf explants under these conditions could be used as a possible micropropagation system for this species. [Thi TMN, Thwe AA, Tuan PA, Chae SC, Park SU. Shoot Organogenesis and Plant Regeneration from Leaf Culture of Rehmannia elata L. Life Sci J 2012;9(4):882-885] (ISSN:1097-8135). http://www.lifesciencesite.com. 136